Application
Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 μg/ml.Application
Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 μg/ml.Application
Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PHLPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHLPP1 Antibody: SCOP, PHLPP, PLEKHE1, KIAA0606, SCOP, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1 |
| Product description | PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.1) Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30. |
| Immunogen | PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHLPP1 |
|---|---|
| Alternative Names | PHLPP1 Antibody: SCOP PHLPP PLEKHE1 KIAA0606 SCOP PH domain leucine-rich repeat-containing protein phosphatase 1 Pleckstrin homology domain-containing family E member 1 PH domain-containing family E member 1 |
| Molecular Weight(MW) | 189 kDa |
| Tissue Specificity | At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2. |
Application
Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 μg/ml.
Application
Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 μg/ml.
Application
Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
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| Application Notes | PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | PHLPP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
