Application
Immunocytochemistry of PHLPP2 in SW480 cells with PHLPP2 antibody at 2.5 μg/ml.Application
Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.Application
Immunofluorescence of PHLPP2 in SW480 cells with PHLPP2 antibody at 5 μg/ml.| Product Name | PHLPP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHLPP2 Antibody: PHLPPL, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like |
| Product description | PHLPP2 Antibody: PHLPP2 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. PHLPP2 associates with and is inhibited by adenylyl cyclase type 6 (AC6), thereby allowing Akt activation.1) Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30. |
| Immunogen | PHLPP2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2. The immunogen is located within the last 50 amino acids of PHLPP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PHLPP2 antibody can be used for detection of PHLPP2 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHLPP2 |
|---|---|
| Alternative Names | PHLPP2 Antibody: PHLPPL KIAA0931 PHLPPL PH domain leucine-rich repeat-containing protein phosphatase 2 PH domain leucine-rich repeat-containing protein phosphatase-like PHLPP-like |
| Molecular Weight(MW) | 156 kDa |
| Tissue Specificity | At least three isoforms are known to exist; this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1. |
Application
Immunocytochemistry of PHLPP2 in SW480 cells with PHLPP2 antibody at 2.5 μg/ml.
Application
Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
Application
Immunofluorescence of PHLPP2 in SW480 cells with PHLPP2 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
|---|---|
| Application Notes | PHLPP2 antibody can be used for detection of PHLPP2 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHLPP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PHLPP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
