Application
Western blot analysis of PHOX2B in 293 cell lysate with PHOX2B antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PHOX2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHOX2B Antibody: PMX2B, NBLST2, NBPhox, PMX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox |
| Product description | PHOX2B Antibody: PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.1) Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94 |
| Immunogen | PHOX2B antibody was raised against a 15 amino acid synthetic peptide near the center of human PHOX2B. The immunogen is located within amino acids 130 - 180 of PHOX2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PHOX2B antibody can be used for detection of PHOX2B by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHOX2B |
|---|---|
| Alternative Names | PHOX2B Antibody: PMX2B NBLST2 NBPhox PMX2B Paired mesoderm homeobox protein 2B Neuroblastoma Phox |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (75%), Bovine: (79%) |
| Tissue Specificity | PHOX2B antibody is predicted to not cross-react with other paired homeobox family members. |
Application
Western blot analysis of PHOX2B in 293 cell lysate with PHOX2B antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | PHOX2B antibody can be used for detection of PHOX2B by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHOX2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PHOX2B Antibody is supplied in PBS containing 0.02% sodium azide. |
