Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PHTNS Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)| Product Name | PHTNS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phostensin, Protein phosphatase 1 F-actin cytoskeleton-targeting subunit, Protein phosphatase 1 regulatory subunit 18, PPP1R18, HKMT1098, KIAA1949 |
| Product description | PHTNS interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton。1) Lai, N.S., et al. Biochem. Biophys. Res. Commun. 387(4):676-681(2009) |
| Immunogen | This PHTNS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the N-terminal region of human PHTNS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1R18 |
|---|---|
| Alternative Names | Phostensin Protein phosphatase 1 F-actin cytoskeleton-targeting subunit Protein phosphatase 1 regulatory subunit 18 PPP1R18 HKMT1098 KIAA1949 |
| Molecular Weight(MW) | 68 kDa |
Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PHTNS Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PHTNS Antibody in Ramos cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
