Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PHYH using rabbit polyclonal PHYH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PHYH Antibody in MDA-MB435 cell line lysates (35ug/lane)| Product Name | PHYH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phytanoyl-CoA dioxygenase, peroxisomal, Phytanic acid oxidase, Phytanoyl-CoA alpha-hydroxylase, PhyH, PHYH, PAHX |
| Product description | PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.1) Foulon,V., et.al., J. Biol. Chem. 280 (11), 9802-9812 (2005) |
| Immunogen | This PHYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human PHYH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHYH |
|---|---|
| Alternative Names | Phytanoyl-CoA dioxygenase peroxisomal Phytanic acid oxidase Phytanoyl-CoA alpha-hydroxylase PhyH PHYH PAHX |
| Molecular Weight(MW) | 39 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PHYH using rabbit polyclonal PHYH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PHYH Antibody in MDA-MB435 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
