Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467).| Product Name | PI3K P85 (phospho Tyr467) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p85, AGM7, GRB1, p85-ALPHA, LHR, MDU2, MDU3, MIC4, PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha |
| Product description | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.1) Stoyanov, B. et al. (1995) Science 269, 690-3. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. Neri, L. M. et al. (2002) Biochim Biophys Acta 1584, 73-80. |
| Immunogen | PI3K P85 (phospho-Tyr467) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 467 (L-Y (p) -E-E-Y) derived from Human PI3K P85. |
| Modification | p-Tyr467 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R1 |
|---|---|
| Alternative Names | p85 AGM7 GRB1 p85-ALPHA LHR MDU2 MDU3 MIC4 PI3-kinase p85 subunit alpha PtdIns-3-kinase p85-alpha |
| Molecular Weight(MW) | 85 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PI3K P85 only when phosphorylated at tyrosine 467. |
| Entrez Gene | 5295 |
|---|---|
| Protein Accession | NP_852556.2 / NP_001107644.1 / NP_005018.1 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
