Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PI3KCB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI3KCB antibody detected the PI3KCB protein (arrow).| Product Name | PI3KCB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subuni |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.1) Brock, C., et al., J. Cell Biol. 160(1):89-99 (2003). |
| Immunogen | This PI3KCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713-744 amino acids from the C-terminal region of human PI3KCB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PIK3CB |
|---|---|
| Alternative Names | Phosphatidylinositol 4 5-bisphosphate 3-kinase catalytic subunit beta isoform PI3-kinase subunit beta PI3K-beta PI3Kbeta PtdIns-3-kinase subunit beta Phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta PtdIns-3-kinase subuni |
| Molecular Weight(MW) | 123 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PI3KCB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI3KCB antibody detected the PI3KCB protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288037