Application
P immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of P for immunohistochemistry.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).Application
Western blot analysis of PIK3CB using rabbit polyclonal P using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.| Product Name | PIK3CB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subuni |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. |
| Immunogen | This PIK3CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-154 amino acids from the N-terminal region of human PIK3CB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3CB |
|---|---|
| Alternative Names | Phosphatidylinositol 4 5-bisphosphate 3-kinase catalytic subunit beta isoform PI3-kinase subunit beta PI3K-beta PI3Kbeta PtdIns-3-kinase subunit beta Phosphatidylinositol 4 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta PtdIns-3-kinase subuni |
| Molecular Weight(MW) | 123 kDa |
Application
P immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of P for immunohistochemistry.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).
Application
Western blot analysis of PIK3CB using rabbit polyclonal P using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288097