Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
Product description
Phosphatidylinositolpolyphosphates (PtdInsPs) are
centrally involved in many biologic processes, ranging from cell
growth and organization of the actin cytoskeleton to endo- and
exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an
essential step in the biosynthesis of PtdInsPs (Barylko et al.,
2001 [PubMed 11244087]).1) Li, J., et al. Oncogene 29(17):2550-2559(2010)
Immunogen
This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A.
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.