Application
Immunofluorescence of PICALM in human brain tissue with PICALM antibody at 20 μg/mL.Application
Western blot analysis of PICALM in EL4 cell lysate with PICALM antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PICALM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PICALM Antibody: LAP, CALM, CLTH, Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein |
| Product description | PICALM Antibody: PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).1) Tebar F, Bohlander SK, and Sorkin A. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin mediated traffic. Mol. Biol. Cell1999; 10:2687-702. |
| Immunogen | PICALM antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PICALM. The immunogen is located within the first 50 amino acids of PICALM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PICALM antibody can be used for detection of PICALM by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PICALM |
|---|---|
| Alternative Names | PICALM Antibody: LAP CALM CLTH Phosphatidylinositol-binding clathrin assembly protein Clathrin assembly lymphoid myeloid leukemia protein |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of PICALM in human brain tissue with PICALM antibody at 20 μg/mL.
Application
Western blot analysis of PICALM in EL4 cell lysate with PICALM antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | PICALM antibody can be used for detection of PICALM by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PICALM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PICALM Antibody is supplied in PBS containing 0.02% sodium azide. |
