Application
Immunocytochemistry of PIERCE1 in A20 cells with PIERCE1 antibody at 2.5 μg/mL.Application
Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PIERCE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIERCE1 Antibody: PIERCE1, RbEST47, UPF0691 protein C9orf116, p53-induced expression in RB-null cells protein 1, Pierce1 |
| Product description | PIERCE1 Antibody: PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation. PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer. PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene. It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.1) Sung YH, Kim HJ, Devkota S, et al. Pierce1, a novel p53 target gene contributing to the ultraviolet-induced DNA damage response. Cancer Res. 2010; 70:10454-63. |
| Immunogen | PIERCE1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human PIERCE1. The immunogen is located within the first 50 amino acids of PIERCE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C9orf116 |
|---|---|
| Alternative Names | PIERCE1 Antibody: PIERCE1 RbEST47 UPF0691 protein C9orf116 p53-induced expression in RB-null cells protein 1 Pierce1 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | PIERCE1 antibody is predicted to not cross-react with other UPF0691 family members. |
Application
Immunocytochemistry of PIERCE1 in A20 cells with PIERCE1 antibody at 2.5 μg/mL.
Application
Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIERCE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIERCE1 Antibody is supplied in PBS containing 0.02% sodium azide. |
