Application
Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.Application
Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.Application
Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.| Product Name | PIG-Y Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIG-Y Antibody: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y |
| Product description | PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.1) Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. Bioessays2003; 25:367-85. |
| Immunogen | PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y. The immunogen is located within the last 50 amino acids of PIG-Y. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIG-Y antibody can be used for detection of PIG-Y by Western blot at 5 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIGY |
|---|---|
| Alternative Names | PIG-Y Antibody: PIG-Y Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y Phosphatidylinositol-glycan biosynthesis class Y protein PIG-Y |
| Molecular Weight(MW) | Predicted: 8 kDa Observed: 9 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This antibody only detects isoform 1 of PIG-Y. |
Application
Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.
Application
Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.
Application
Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1375 – Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | PIG-Y antibody can be used for detection of PIG-Y by Western blot at 5 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIG-Y antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide. |
