Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
PIGA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PIGA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A, PIGA |
| Product description | This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.1) Borowitz, M.J., et al. CytometryClin Cytom 78(4):211-230(2010) |
| Immunogen | This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIGA |
|---|---|
| Alternative Names | Phosphatidylinositol N-acetylglucosaminyltransferase subunit A GlcNAc-PI synthesis protein Phosphatidylinositol-glycan biosynthesis class A protein PIG-A PIGA |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
PIGA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
