Application
Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PIGM Antibody in T47D cell line lysates (35ug/lane)Application
PIGM Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PIGM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPI mannosyltransferase 1, 241-, GPI mannosyltransferase I, GPI-MT-I, Phosphatidylinositol-glycan biosynthesis class M protein, PIG-M, PIGM |
| Product description | PIGM encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. PIGM encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum.1) Almeida, A.M., et al. Nat. Med. 12(7):846-851(2006) Maeda, Y., et al. EMBO J. 20 (1-2), 250-261 (2001) Kinoshita, T., et al. Curr Opin Chem Biol 4(6):632-638(2000) |
| Immunogen | This PIGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human PIGM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIGM |
|---|---|
| Alternative Names | GPI mannosyltransferase 1 241- GPI mannosyltransferase I GPI-MT-I Phosphatidylinositol-glycan biosynthesis class M protein PIG-M PIGM |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PIGM Antibody in T47D cell line lysates (35ug/lane)
Application
PIGM Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
