Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PIK3R1 using rabbit polyclonal PIK3R1 Antibody (Y580) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).| Product Name | PIK3R1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alph |
| Product description | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.1) Kobayashi, H., et al., J. Biol. Chem. 279(8):6371-6379 (2004). |
| Immunogen | This PIK3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 558-587 amino acids from human PIK3R1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R1 |
|---|---|
| Alternative Names | Phosphatidylinositol 3-kinase regulatory subunit alpha PI3-kinase regulatory subunit alpha PI3K regulatory subunit alpha PtdIns-3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PI3-kinase subunit p85-alph |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PIK3R1 using rabbit polyclonal PIK3R1 Antibody (Y580) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
