Application
Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.Application
Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.Application
Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | PIK3R4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15 |
| Product description | PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).1) Herman PK, Stack JH, and Emr SD. A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J. 1991; 10:4049-60. |
| Immunogen | PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4. The immunogen is located within amino acids 1290 - 1340 of PIK3R4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R4 |
|---|---|
| Alternative Names | Phosphoinositide-3-kinase regulatory subunit 4 p150 Phosphoinositide 3-kinase adaptor protein PI3-kinase p150 subunit PI3-kinase regulatory subunit 4 VPS15 |
| Molecular Weight(MW) | Predicted: 149 kDa Observed: 140 kDa |
| Tissue Specificity | PIK3R4 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.
Application
Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.
Application
Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1315 - Human Tonsil Tissue Lysate |
|---|---|
| Application Notes | PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIK3R4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide. |
