Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PIK4CB Antibody in Hela cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PIK4CB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KB, PIK4CB |
| Product description | PIK4CB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.1) Jeganathan,S., et.al., Mol. Cell. Biol. 28 (14), 4549-4561 (2008) Pizarro-Cerda,J., et.al., Cell. Microbiol.(10), 2381-2390 (2007) |
| Immunogen | This PIK4CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the Central region of human PIK4CB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PI4KB |
|---|---|
| Alternative Names | Phosphatidylinositol 4-kinase beta PI4K-beta PI4Kbeta PtdIns 4-kinase beta NPIK PI4K92 PI4KB PIK4CB |
| Molecular Weight(MW) | 91 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PIK4CB Antibody in Hela cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
