Application
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Pim-1 (phospho Tyr309) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1 |
| Product description | Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.1) Selten G., Cell 46:603-611(1986). |
| Immunogen | Pim-1 (phospho Tyr309) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 309 (A-A-V (p)-W-S) derived from Human Pim-1. |
| Modification | p-Tyr309 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIM1 |
|---|---|
| Alternative Names | PIM-1 Proto-oncogene serine/threonine-protein kinase pim-1 EC 2.7.11.1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309. |
| Entrez Gene | 5292 |
|---|---|
| Protein Accession | NP_001230115.1 |
Application
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
