Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.Application
Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PIM3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase pim-3, PIM3 |
| Product description | PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.1) Mikkers, H., et al., Mol. Cell. Biol. 24(13):6104-6115 (2004). Eichmann, A., et al., Oncogene 19(9):1215-1224 (2000). |
| Immunogen | This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PIM3 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase pim-3 PIM3 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Application
Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
