Application
Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Pin1 (phospho Ser16) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PPIase Pin1, Rotamase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2.1.8, |
| Product description | Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.1) Lee YC, et al. (2013)J Cell Sci 126, 4862-72. Ando K, et al. (2013)Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013)Mol Cell Biol 33, 48-58. |
| Immunogen | Pin1 (phospho Ser16) antibody was raised against a peptide sequence around phosphorylation site of serine16 (R-M-S (p)-R-S) derived from Human Pin1 . |
| Modification | p-Ser16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIN1 |
|---|---|
| Alternative Names | PPIase Pin1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 EC 5.2.1.8 |
| Molecular Weight(MW) | 18 kDa |
| Tissue Specificity | The antibody detects endogenous level of Pin1 only when phosphorylated at serine 16. |
| Entrez Gene | 5300 |
|---|---|
| Protein Accession | NP_006212.1 |
Application
Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
