Application
Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.Application
Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.Application
Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.| Product Name | PION Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PION Antibody: PION, PION, Gamma-secretase-activating protein, Protein pigeon homolog, GSAP |
| Product description | PION Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease.1) Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature1988; 331:525-77. |
| Immunogen | PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION. The immunogen is located within amino acids 770 - 820 of PION. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PION |
|---|---|
| Alternative Names | PION Antibody: PION PION Gamma-secretase-activating protein Protein pigeon homolog GSAP |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members. |
Application
Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.
Application
Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.
Application
Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PION antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PION Antibody is supplied in PBS containing 0.02% sodium azide. |
