Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | PIR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIR, |
| Product description | PIR is a member of the cupin superfamily. The protein is an Fe (II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe (II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.1) Adams, M. (2005) J. Biol. Chem. 280 (31), 28675-28682. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PIR antibody can be used for detection of PIR by ELISA at 1:62500. PIR antibody can be used for detection of PIR by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIR |
|---|---|
| Alternative Names | PIR |
| Molecular Weight(MW) | 32 kDa, 32 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PIR antibody can be used for detection of PIR by ELISA at 1:62500. PIR antibody can be used for detection of PIR by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PIR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
