PITX1 is a member of the RIEG/PITX homeobox family, which is involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Please see the Entrez Gene record to access additional publications.1) Chen, Y.N., (2008) World J. Gastroenterol. 14 (2), 292-297.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PITX1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PITX1 antibody can be used for detection of PITX1 by ELISA at 1:62500. PITX1 antibody can be used for detection of PITX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HeLa cells at 1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
PITX1 antibody can be used for detection of PITX1 by ELISA at 1:62500. PITX1 antibody can be used for detection of PITX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PITX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.