Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PITX1 using rabbit polyclonal PITX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.Application
PITX1 Antibody (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | PITX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1, PITX1, BFT, PTX1 |
| Product description | PITX1 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.1) Grimminger,P., Ling,F.C. Anticancer Res. 29 (4), 1281-1286 (2009) |
| Immunogen | This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human PITX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PITX1 |
|---|---|
| Alternative Names | Pituitary homeobox 1 Hindlimb-expressed homeobox protein backfoot Homeobox protein PITX1 Paired-like homeodomain transcription factor 1 PITX1 BFT PTX1 |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PITX1 using rabbit polyclonal PITX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.
Application
PITX1 Antibody (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PITX1 antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
