Application
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.Application
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.Application
Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | PIWI-L1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1 |
| Product description | PIWI-L1 Antibody: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.1) Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol.2009; 25:355-76. |
| Immunogen | PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1. The immunogen is located within amino acids 60 - 110 of PIWI-L1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIWIL1 |
|---|---|
| Alternative Names | PIWI-L1 Antibody: HIWI MIWI PIWI CT80.1 HIWI Piwi-like protein 1 |
| Molecular Weight(MW) | Predicted: 85, 90 kDa Observed: 95 kDa |
| Sequence Similarities | 2) Cat. No. 1211 - HepG2 Cell Lysate |
Application
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.
Application
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.
Application
Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIWI-L1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide. |
