Application
Immunocytochemistry of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 2.5 μg/ml.Application
Immunofluorescence of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 5 μg/ml.Application
Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.| Product Name | PIWI-L2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIWI-L2 Antibody: CT80, HILI, mili, PIWIL1L, Piwi-like protein 2, CT80 |
| Product description | PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.1) Kuramochi-Miyagawa S, Kimura T, Yomogida K, et al. Two mouse piwi-related genes: miwi and mili. Mech. Dev. 108:121-33 |
| Immunogen | PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410 - 460 of PIWI-L2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIWIL2 |
|---|---|
| Alternative Names | PIWI-L2 Antibody: CT80 HILI mili PIWIL1L Piwi-like protein 2 CT80 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members. |
| Entrez Gene | 55124 |
|---|---|
| Protein Accession | NP_00129193 |
Application
Immunocytochemistry of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 2.5 μg/ml.
Application
Immunofluorescence of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 5 μg/ml.
Application
Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIWI-L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide. |
