Application
Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 μg/mL.Application
Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PIWI-L3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIWI-L3 Antibody: HIWI3, Piwi-like protein 3 |
| Product description | PIWI-L3 Antibody: PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. Recent studies have shown that overexpression of PIWI-L3 (as well as other PIWI-like proteins) are potential biomarkers for astrocytic glioma, meningioma, and other cancers.1) Sasaki T, Shiohama A, Minoshima S, et al. Identification of eight members of the Argonaute family in the human genome small star, filled. Genomics2003; 323-30. |
| Immunogen | PIWI-L3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3. The immunogen is located within amino acids 130 - 180 of PIWI-L3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIWIL3 |
|---|---|
| Alternative Names | PIWI-L3 Antibody: HIWI3 Piwi-like protein 3 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Entrez Gene | 440822 |
|---|---|
| Protein Accession | NP_001008496 |
Application
Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 μg/mL.
Application
Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIWI-L3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIWI-L3 Antibody is supplied in PBS containing 0.02% sodium azide. |
