Application
Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 μg/ml.Application
Immunohistochemistry of PJA1 in mouse brain tissue with PJA1 antibody at 5 μg/ml.Application
Western blot analysis of PJA1 in human brain tissue lysate with PJA1 antibody at (A) 0.25 and (B) 0.5 μg/ml.| Product Name | PJA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70 |
| Product description | Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).1) Yu P, Chen Y, Tagle DA, et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics 2002; 79:869-74. |
| Immunogen | PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PJA1 |
|---|---|
| Alternative Names | Praja ring finger 1 E3 ubiquitin-protein ligase Praja-1 PRAJA1 RING finger protein 70 RNF70 |
| Molecular Weight(MW) | Predicted: 71 kDa Observed: 71 kDa |
| Tissue Specificity | PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms. |
Application
Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 μg/ml.
Application
Immunohistochemistry of PJA1 in mouse brain tissue with PJA1 antibody at 5 μg/ml.
Application
Western blot analysis of PJA1 in human brain tissue lysate with PJA1 antibody at (A) 0.25 and (B) 0.5 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 – 0.5 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PJA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PJA1 antibody is supplied in PBS containing 0.02% sodium azide. |
