Application
Western blot analysis of PRKCB using PKC beta1/2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human brain lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | PKC beta1/2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1 |
| Product description | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.1) Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004). |
| Immunogen | This PKC beta1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-334 amino acids from the Central region of human PKC beta1/2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCB |
|---|---|
| Alternative Names | Protein kinase C beta type PKC-B PKC-beta PRKCB PKCB PRKCB1 |
| Molecular Weight(MW) | 77 kDa |
Application
Western blot analysis of PRKCB using PKC beta1/2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human brain lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
