Application
Western blot analysis of lysed extracts from PC12 cells using PKCΘ (Ab-676).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Ab-676). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKCΘ (Ab-676).| Product Name | PKCtheta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKCT, nPKC-theta, MOR1 |
| Product description | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-κ-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.1) Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253. Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123. Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119. |
| Immunogen | PKCtheta (Ab-676) antibody was raised against a peptide sequence around aa. 674~678 (R-L-S-F-A) derived from Human PKCΘ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCQ |
|---|---|
| Alternative Names | PRKCT nPKC-theta MOR1 |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PKCΘ protein. |
| Entrez Gene | 5588 |
|---|---|
| Protein Accession | NP_006248.1 |
Application
Western blot analysis of lysed extracts from PC12 cells using PKCΘ (Ab-676).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Ab-676). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKCΘ (Ab-676).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
