Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Phospho-Ser695).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using PKCΘ (Phospho-Ser695).| Product Name | PKCtheta (phospho Ser695) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKCT, nPKC-theta, MMAC1, TEP1, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT |
| Product description | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-κ-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.1) Xu ZB, et al. (2004) J Biol Chem 279:50401-50409Thebault S, et al. (2004) Mol Immunol 40: 931-942 |
| Immunogen | PKCtheta (Phospho-Ser695) antibody was raised against a peptide sequence around phosphorylation site of serine 695 (N-F-S (p) -F-M) derived from Human PKCΘ. |
| Modification | p-Ser695 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCQ |
|---|---|
| Alternative Names | PRKCT nPKC-theta MMAC1 TEP1 KPCT PKC-theta PKCQ PRKCQ PRKCT |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of PKCΘ only when phosphorylated at serine 695. |
| Entrez Gene | 5588 |
|---|---|
| Protein Accession | NP_006248.1 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCΘ (Phospho-Ser695).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using PKCΘ (Phospho-Ser695).| Application Notes | Western Blot: 1:500, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
