Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate| Product Name | PKN beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase N3, Protein kinase PKN-beta, Protein-kinase C-related kinase 3, PKN3, PKNBETA |
| Product description | PKN3, a protein kinase C-related molecule, mediates malignant cell growth downstream of activated PI3K. PKN3 is required for invasive prostate cell growth as assessed by 3D cell culture assays and in an orthotopic mouse tumor model by inducible expression of short hairpin RNA (shRNA). PKN3 is regulated by PI3K at both the expression level and the catalytic activity level. Therefore, PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of PI3K.1) Oishi, K., et al., Biochem. Biophys. Res. Commun. 261(3):808-814 (1999). |
| Immunogen | This PKN beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463-492 amino acids from the C-terminal region of human PKN beta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PKN3 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase N3 Protein kinase PKN-beta Protein-kinase C-related kinase 3 PKN3 PKNBETA |
| Molecular Weight(MW) | 99 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
