Application
Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.Application
Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PLA1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLA1A Antibody: PSPLA1, PS-PLA1, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1 |
| Product description | PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.1) Sato T, Aoki J, Nagai Y, et al. Serine phospholipid-specific phospholipase A that is secreted from activated platelets. A new member of the lipase family. J. Biol. Chem. 1997; 272:2192-8. |
| Immunogen | PLA1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human PLA1A. The immunogen is located within amino acids 380 - 430 of PLA1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLA1A |
|---|---|
| Alternative Names | PLA1A Antibody: PSPLA1 PS-PLA1 NMD PSPLA1 Phospholipase A1 member A Phosphatidylserine-specific phospholipase A1 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms. |
Application
Immunofluorescence of PLA1A in human kidney tissue with PLA1A antibody at 20 μg/mL.
Application
Western blot analysis of PLA1A in human kidney tissue lysate with PLA1A antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
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| Application Notes | PLA1A antibody can be used for detection of PLA1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PLA1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PLA1A Antibody is supplied in PBS containing 0.02% sodium azide. |
