Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PLA2G1B using rabbit polyclonal PLA2G1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.Application
Western blot analysis of PLA2G1B Antibody in mouse spleen tissue lysates (35ug/lane)| Product Name | PLA2G1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phospholipase A2, Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolase 1B, PLA2G1B, PLA2, PLA2A, PPLA2 |
| Product description | PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets1) Xu, W., et al. J. Biol. Chem. 284(24):16659-16666(2009) |
| Immunogen | This PLA2G1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the C-terminal region of human PLA2G1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLA2G1B |
|---|---|
| Alternative Names | Phospholipase A2 Group IB phospholipase A2 Phosphatidylcholine 2-acylhydrolase 1B PLA2G1B PLA2 PLA2A PPLA2 |
| Molecular Weight(MW) | 16 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PLA2G1B using rabbit polyclonal PLA2G1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.
Application
Western blot analysis of PLA2G1B Antibody in mouse spleen tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
