Application
Immunohistochemistry staining of PLA2G3 in colon carcinoma tissue using PLA2G3 Antibody.| Product Name | PLA2G3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLA2G3, GIII-SPLA2, Phospholipase A2, group III, SPLA2-III, GIII sPLA2, SPLA2III |
| Product description | Human group III secreted phospholipase A2 belongs to a superfamily of intracellular and secreted enzymes that catalyze the hydrolysis of glycerophospholipids at the sn-2 position to release fatty acids and lysophospholipids. Group III PLA2 enzymes were originally identified in invertebrates such as bees and scorpions. Human group III sPLA2 has been shown to be a calcium dependent enzyme. It consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains that are proteolytically removed in most tissues. The sPLA2 domain is sufficient for catalytic activity. SPLA2-III is preferentially expressed in the microvascular endothelium of tissues with inflammation, ischemic injury and cancer. |
| Immunogen | PLA2G3 antibody was raised against a peptide located near the internal domain of PLA2G3 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| PLA2G3 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 1:200 - 1:400.: |
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| Species Reactivity | GibbonGorillaHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLA2G3 |
|---|---|
| Alternative Names | PLA2G3 GIII-SPLA2 Phospholipase A2 group III SPLA2-III GIII sPLA2 SPLA2III |
| Tissue Specificity | BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Application
Immunohistochemistry staining of PLA2G3 in colon carcinoma tissue using PLA2G3 Antibody.| Application Notes | PLA2G3 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 1:200 - 1:400.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | PLA2G3 antibody should be stored long term (months) at -80 ˚C and short term (days) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
