Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PLA2G6 Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
PLA2G6 Antibody IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PLA2G6 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | PLA2G6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 85/88 kDa calcium-independent phospholipase A2, CaI-PLA2, Group VI phospholipase A2, GVI PLA2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, iPLA2-beta, Patatin-like phospholipase domain-containing protein 9, PNPLA9, PLA2G6, |
| Product description | The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.1) Ayilavarapu, S., et al. J. Immunol. 184(3):1507-1515(2010) Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010) Tan, E.K., et al. Ann. Neurol. 67 (1), 148 (2010) Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009) |
| Immunogen | This PLA2G6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-580 amino acids from the Central region of human PLA2G6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PLA2G6 |
|---|---|
| Alternative Names | 85/88 kDa calcium-independent phospholipase A2 CaI-PLA2 Group VI phospholipase A2 GVI PLA2 Intracellular membrane-associated calcium-independent phospholipase A2 beta iPLA2-beta Patatin-like phospholipase domain-containing protein 9 PNPLA9 PLA2G6 |
| Molecular Weight(MW) | 90 kDa |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PLA2G6 Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
PLA2G6 Antibody IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PLA2G6 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
