Application
Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.Application
Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.Application
Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PLAC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLAC3 Antibody: PAPPE, PLAC3, PAPP-E, PAPP-A2, Pappalysin-2, Pregnancy-associated plasma protein A2 |
| Product description | PLAC3 Antibody: PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a lesser extent IGFBP-3 produced in the maternal decidua promotes feto-placental growth. Recent studies have shown elevated levels of PLAC3 in hypertensive disorders of pregnancy including preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.1) Overgaard MT, Boldt HB, Laursen LS, et al. Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J. Biol. Chem.2001; 276:1849-53. |
| Immunogen | PLAC3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human PLAC3. The immunogen is located within amino acids 450 - 500 of PLAC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAPPA2 |
|---|---|
| Alternative Names | PLAC3 Antibody: PAPPE PLAC3 PAPP-E PAPP-A2 Pappalysin-2 Pregnancy-associated plasma protein A2 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.
Application
Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.
Application
Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PLAC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PLAC3 Antibody is supplied in PBS containing 0.02% sodium azide. |
