Application
Immunocytochemistry of PLAC4 in HeLa cells with PLAC4 antibody at 10 μg/mL.Application
Immunofluorescence of PLAC4 in HeLa cells with PLAC4 antibody at 20 μg/mL.Application
Western blot analysis of PLAC4 in human placenta tissue lysate with PLAC4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PLAC4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLAC4 Antibody: PRED78, C21orf115, D21S418E, PP1416, Placenta-specific protein 4 |
| Product description | PLAC4 Antibody: PLAC4 (placenta-specific protein 4), was initially discovered as a gene whose mRNA was expressed in placental syncytiotrophoblast and choriocarcinoma cells. While the function of PLAC4 is currently unclear, its gene is known to be located on chromosome 21q22.3. Due to the PLAC4 gene location on chromosome 21 and the release of its mRNA transcripts into maternal bloodstream from the placenta, it has been proposed to be a useful marker noninvasive prenatal chromosome 21 aneuploidy detection, although other reports suggests that PLAC4 mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21.1) Kido S, Sakuragi N, Bronner MP, et al. D21S418E identifies a cAMP-regulated gene located on chromosome 21q22.3 that is expressed in placental syncytiotrophoblast and choriocarcinoma. Genomics1993; 17:256-9. |
| Immunogen | PLAC4 antibody was raised against a 17 amino acid synthetic peptide near the center of human PLAC4. The immunogen is located within amino acids 20 - 70 of PLAC4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PLAC4 antibody can be used for detection of PLAC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLAC4 |
|---|---|
| Alternative Names | PLAC4 Antibody: PRED78 C21orf115 D21S418E PP1416 Placenta-specific protein 4 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of PLAC4 in HeLa cells with PLAC4 antibody at 10 μg/mL.
Application
Immunofluorescence of PLAC4 in HeLa cells with PLAC4 antibody at 20 μg/mL.
Application
Western blot analysis of PLAC4 in human placenta tissue lysate with PLAC4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1309 - Human Placenta Tissue Lysate |
|---|---|
| Application Notes | PLAC4 antibody can be used for detection of PLAC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PLAC4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PLAC4 Antibody is supplied in PBS containing 0.02% sodium azide. |
