Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PLCL1 Antibody in Jurkat cell line lysates (35ug/lane)| Product Name | PLCL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inactive phospholipase C-like protein 1, PLC-L1, Phospholipase C-deleted in lung carcinoma, Phospholipase C-related but catalytically inactive protein, PRIP, PLCL1 |
| Product description | PLCL1 is an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and sphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a nhibitor of PPP1C.1) Liu,Y.Z., et.al., PLoS ONE(9), E3160 (2008) |
| Immunogen | This PLCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-176 amino acids from the N-terminal region of human PLCL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCL1 |
|---|---|
| Alternative Names | Inactive phospholipase C-like protein 1 PLC-L1 Phospholipase C-deleted in lung carcinoma Phospholipase C-related but catalytically inactive protein PRIP PLCL1 |
| Molecular Weight(MW) | 123 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PLCL1 Antibody in Jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
