Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PLCL2 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
PLCL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | PLCL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inactive phospholipase C-like protein 2, PLC-L(2), PLC-L2, Phospholipase C-L2, Phospholipase C-epsilon-2, PLC-epsilon-2, PLCL2, KIAA1092, PLCE2 |
| Product description | PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).1) Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009) |
| Immunogen | This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1099-1126 amino acids from the C-terminal region of human PLCL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCL2 |
|---|---|
| Alternative Names | Inactive phospholipase C-like protein 2 PLC-L(2) PLC-L2 Phospholipase C-L2 Phospholipase C-epsilon-2 PLC-epsilon-2 PLCL2 KIAA1092 PLCE2 |
| Molecular Weight(MW) | 126 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PLCL2 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
PLCL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
