Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.| Product Name | PLEKHF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pleckstrin homology domain-containing family F member 1, PH domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, Apoptosis-inducing protein, PH and FYVE domain-containing protein 1, Phafin-1, Z |
| Product description | PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.1) Chen, W., et al. J. Biol. Chem. 280(49):40985-40995(2005) |
| Immunogen | This PLEKHF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human PLEKHF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLEKHF1 |
|---|---|
| Alternative Names | Pleckstrin homology domain-containing family F member 1 PH domain-containing family F member 1 Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains Apoptosis-inducing protein PH and FYVE domain-containing protein 1 Phafin-1 Z |
| Molecular Weight(MW) | 31 kDa |
Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
