Application
Immunofluorescence of PLKHM1 in human lung tissue with PLKHM1 antibody at 20 μg/mL.Application
Immunohistochemistry of PLEKHM1 in human lung tissue with PLEKHM1 antibody at 5 μg/mL.Application
Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 μg/mL.| Product Name | PLEKHM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLEKHM1 Antibody: B2, AP162, OPTB6, KIAA0356, Pleckstrin homology domain-containing family M member 1, 162 kDa adapter protein, PH domain-containing family M member 1 |
| Product description | PLEKHM1 Antibody: PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.1) Van Wesenbeeck L, Odgren PR, Mackay CA, et al. Localization of the gene causing the osteopetrotic phenotype in the incisors absent (ia) rat on chromosome 10q32.1. J. Bone Miner. Res.2004; 19:183-9. |
| Immunogen | PLEKHM1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human PLEKHM1. The immunogen is located within amino acids 50 - 100 of PLEKHM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLEKHM1 |
|---|---|
| Alternative Names | PLEKHM1 Antibody: B2 AP162 OPTB6 KIAA0356 Pleckstrin homology domain-containing family M member 1 162 kDa adapter protein PH domain-containing family M member 1 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Immunofluorescence of PLKHM1 in human lung tissue with PLKHM1 antibody at 20 μg/mL.
Application
Immunohistochemistry of PLEKHM1 in human lung tissue with PLEKHM1 antibody at 5 μg/mL.
Application
Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
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| Application Notes | PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PLEKHM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PLEKHM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
