Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.Application
Immunofluorescence of PLKHM3 in human brain tissue with PLKHM3 antibody at 20 μg/mL.Application
Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PLEKHM3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLEKHM3 Antibody: DAPR, PLEKHM1L, Pleckstrin homology domain-containing family M member 3, PH domain-containing family M member 3 |
| Product description | PLEKHM3 Antibody: PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.1) Virtanen C, Paris J, and Takahashi M. Identification and characterization of a novel gene, dapr, involved in skeletal muscle differentiation and protein kinase B signaling. J. Biol. Chem.2009; 284:1636-43. |
| Immunogen | PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3. The immunogen is located within the last 50 amino acids of PLEKHM3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLEKHM3 |
|---|---|
| Alternative Names | PLEKHM3 Antibody: DAPR PLEKHM1L Pleckstrin homology domain-containing family M member 3 PH domain-containing family M member 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
Application
Immunofluorescence of PLKHM3 in human brain tissue with PLKHM3 antibody at 20 μg/mL.
Application
Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
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| Application Notes | PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | PLEKHM3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PLEKHM3 Antibody is supplied in PBS containing 0.02% sodium azide. |
