Application
Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B)| Product Name | PLK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, PLK-3, Proliferation-related kinase, PLK3, CNK, FNK, PRK |
| Product description | CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.1) Holtrich, U., et al., Oncogene 19(42):4832-4839 (2000). |
| Immunogen | This PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-646 amino acids from the C-terminal region of human PLK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PLK3 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase PLK3 Cytokine-inducible serine/threonine-protein kinase FGF-inducible kinase Polo-like kinase 3 PLK-3 Proliferation-related kinase PLK3 CNK FNK PRK |
| Molecular Weight(MW) | 72 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
