Application
Western blot analysis in U251 cell line lysates (35ug/lane).Application
Western blot analysis of lysates from A431, Hela, K562, MCF-7, U-87 MG cell line (from left to right), using PLOD1 Antibody at 1:1000 at each lane.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: U-2OS whole cell lysates Lysates/proteins at 20 ug per lane.Application
Antibody staining PLOD1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-RabbitApplication
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(N| Product Name | PLOD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1, LH1, PLOD1, LLH, PLOD |
| Product description | Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.1) Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) : |
| Immunogen | This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000-1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLOD1 |
|---|---|
| Alternative Names | Procollagen-lysine 2-oxoglutarate 5-dioxygenase 1 Lysyl hydroxylase 1 LH1 PLOD1 LLH PLOD |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in U251 cell line lysates (35ug/lane).
Application
Western blot analysis of lysates from A431, Hela, K562, MCF-7, U-87 MG cell line (from left to right), using PLOD1 Antibody at 1:1000 at each lane.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: U-2OS whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Antibody staining PLOD1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit
Application
Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(N| Application Notes | For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
