Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Product Name | PLOD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3, Lysyl hydroxylase 3, LH3, PLOD3 |
| Product description | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.1) Wang, C., et al. J. Cell. Mol. Med. 13(3):508-521(2009) |
| Immunogen | This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLOD3 |
|---|---|
| Alternative Names | Procollagen-lysine 2-oxoglutarate 5-dioxygenase 3 Lysyl hydroxylase 3 LH3 PLOD3 |
| Molecular Weight(MW) | 85 kDa |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
