Application
Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.Application
Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | PMEPA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prostate transmembrane protein androgen induced 1, STAG1, TMEPAI |
| Product description | The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).1) Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63. |
| Immunogen | PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1. The immunogen is located within amino acids 160 - 210 of PMEPA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PMEPA1 |
|---|---|
| Alternative Names | Prostate transmembrane protein androgen induced 1 STAG1 TMEPAI |
| Molecular Weight(MW) | Predicted: 26, 28, 32 kDa Observed: 27 kDa |
| Tissue Specificity | PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms. |
Application
Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.
Application
Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PMEPA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PMEPA1 antibody is supplied in PBS containing 0.02% sodium azide. |
