Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.Application
Antibody used in WB on Human HeLa at 1 ug/ml.Application
Antibody used in IHC on Human Spleen at 5.0 ug/ml.| Product Name | PML Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PML, MYL, PP8675, RNF71, TRIM19 |
| Product description | PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PML. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PML antibody can be used for detection of PML by ELISA at 1:312500. PML antibody can be used for detection of PML by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PML |
|---|---|
| Alternative Names | PML MYL PP8675 RNF71 TRIM19 |
| Molecular Weight(MW) | 59 kDa |
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human HeLa at 1 ug/ml.
Application
Antibody used in IHC on Human Spleen at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PML antibody can be used for detection of PML by ELISA at 1:312500. PML antibody can be used for detection of PML by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PML antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
