Application
Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PNAS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PNAS4 Antibody: DESI, DESI1, DeSI-2, PNAS-4, PPPDE1, CGI-146, FAM152A, C1orf121, Desumoylating isopeptidase 2, PPPDE peptidase domain-containing protein 1 |
| Product description | PNAS4 Antibody: PNAS4 is a highly conserved protein that shares high homology from plants to animals. It was initially identified as a putative apoptosis-related protein in the human acute promyelocytic leukemia cell line NB4. PNAS4 is activated during the early response to DNA damage and can inhibit proliferation via apoptosis when overexpressed in some tumor cells such as U2OS, SKOV3, and A549. PNAS4 inhibits tumor cell proliferation through the following mechanisms: (i) overexpression of PNAS4 causes S phase arrest by regulating the expression of cell cycle-related proteins and (ii) PNAS4 induces apoptosis through the mitochondrial apoptosis pathway. Recent evidence has shown that PNAS4 may be involved in the genesis of some cancers and could be a potential candidate for lung cancer therapy alone or in combination with gemcitabine. At least two isoforms of PNAS4 are known to exist.1) Yao S, Xie L, Qian M, et al. Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation. FEBS Lett.2008; 582:2325-32. |
| Immunogen | PNAS4 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PNAS4. The immunogen is located within the last 50 amino acids of PNAS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PNAS4 antibody can be used for detection of PNAS4 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPPDE1 |
|---|---|
| Alternative Names | PNAS4 Antibody: DESI DESI1 DeSI-2 PNAS-4 PPPDE1 CGI-146 FAM152A C1orf121 Desumoylating isopeptidase 2 PPPDE peptidase domain-containing protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%) |
Application
Western blot analysis of PNAS4 in EL4 cell lysate with PNAS4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | PNAS4 antibody can be used for detection of PNAS4 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PNAS4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PNAS4 Antibody is supplied in PBS containing 0.02% sodium azide. |
