Application
Western blot analysis in Hela,K562 cell line lysates (35ug/lane).Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PNPLA8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium-independent phospholipase A2-gamma, Intracellular membrane-associated calcium-independent phospholipase A2 gamma, iPLA2-gamma, PNPLA-gamma, Patatin-like phospholipase domain-containing protein 8, iPLA2-2, PNPLA8, IPLA22, IPLA2G |
| Product description | PNPLA8 catalyzes cleavage of fatty acids from phospholipids, thereby regulating membrane physical properties and the release of lipid second messengers and growth factors. Phospholipase A2 activity also modulates cellular growth programs, inflammation, and ion channel function.1) Harper, M.T., et al. J. Thromb. Haemost. 6(10):1819-1821(2008) |
| Immunogen | This PNPLA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the N-terminal region of human PNPLA8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNPLA8 |
|---|---|
| Alternative Names | Calcium-independent phospholipase A2-gamma Intracellular membrane-associated calcium-independent phospholipase A2 gamma iPLA2-gamma PNPLA-gamma Patatin-like phospholipase domain-containing protein 8 iPLA2-2 PNPLA8 IPLA22 IPLA2G |
| Molecular Weight(MW) | 88 kDa |
Application
Western blot analysis in Hela,K562 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
